fibroblast medium fm innoprot p60108 human il 6 duoset elisa bio techne dy206 05 r d human il 8 cxcl8 (Innoprot Inc)
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Fibroblast Medium Fm Innoprot P60108 Human Il 6 Duoset Elisa Bio Techne Dy206 05 R D Human Il 8 Cxcl8, supplied by Innoprot Inc, used in various techniques. Bioz Stars score: 93/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 41 article reviews
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Cell Culture:Article Title: An antiageing telomere-protective effect of superoxide dismutase-rich Tetraselmis chui in human cells. Article Snippet: Three aliquots of the supernatant were used for SOD activity determination using a kinetic method with the SOD Assay Kit—WST (Dojindo) according to the manufacturer’s instructions, yielding an average value of 3,545 U/mL. .. Neonatal human fibroblasts (ATCC PCS-201–010TM) were cultured in six-well plates at a seeding density of 5 × 103 cells/cm2 in Article Title: An antiageing telomere-protective effect of superoxide dismutase-rich Tetraselmis chui in human cells. Article Snippet: .. Primary neonatal human fibroblasts (≤ 3 passages) were cultured under standard conditions and seeded in 96-well plates at 5000 cells/well (three days) or 3500 cells/well (one week) using the Single Cell:Article Title: Tumour cell-derived serglycin promotes IL-8 secretion of CAFs in gastric cancer. Article Snippet: Digested cells were filtered through a 40-mm cell strainer (Milex-GP) and centrifuged at 1500 rpm for 10min. .. The single-cell suspension was incubated in a Article Title: Tumour cell-derived serglycin promotes IL-8 secretion of CAFs in gastric cancer Article Snippet: Fresh samples were washed with serum-free DMEM, cut into small pieces, and were transferred to a 0.15% collagenase IV solution, followed by incubation at 37 °C for 40 min. Digested cells were filtered through a 40-mm cell strainer (Milex-GP) and centrifuged at 1500 rpm for 10 min. .. The single-cell suspension was incubated in a Suspension:Article Title: Tumour cell-derived serglycin promotes IL-8 secretion of CAFs in gastric cancer. Article Snippet: Digested cells were filtered through a 40-mm cell strainer (Milex-GP) and centrifuged at 1500 rpm for 10min. .. The single-cell suspension was incubated in a Article Title: Tumour cell-derived serglycin promotes IL-8 secretion of CAFs in gastric cancer Article Snippet: Fresh samples were washed with serum-free DMEM, cut into small pieces, and were transferred to a 0.15% collagenase IV solution, followed by incubation at 37 °C for 40 min. Digested cells were filtered through a 40-mm cell strainer (Milex-GP) and centrifuged at 1500 rpm for 10 min. .. The single-cell suspension was incubated in a Incubation:Article Title: Tumour cell-derived serglycin promotes IL-8 secretion of CAFs in gastric cancer. Article Snippet: Digested cells were filtered through a 40-mm cell strainer (Milex-GP) and centrifuged at 1500 rpm for 10min. .. The single-cell suspension was incubated in a Article Title: Tumour cell-derived serglycin promotes IL-8 secretion of CAFs in gastric cancer Article Snippet: Fresh samples were washed with serum-free DMEM, cut into small pieces, and were transferred to a 0.15% collagenase IV solution, followed by incubation at 37 °C for 40 min. Digested cells were filtered through a 40-mm cell strainer (Milex-GP) and centrifuged at 1500 rpm for 10 min. .. The single-cell suspension was incubated in a |
![Characterisation of primary human peritoneal mesothelial cells (HPMCs) and human peritoneal fibroblasts (HPFs) compared to the LP-9 mesothelial cell line and normal human dermal fibroblasts (NHDFs). (A) Representative phase-contrast micrographs of LP-9, HPMCs, NHDFs and HPFs. Mesothelial and <t>fibroblast</t> cells exhibit cobblestone and spindle-like morphologies, respectively. (B) We observed localised expression of the cytoskeletal markers cytokeratin (CK) and vimentin (VIM) in LP-9 cells and HPMCs, while VIM, but not CK, was expressed in NHDFs and HPFs. HPMCs ( n =6) showed a high percentage of CK + /VIM + cells [98.20±1.05% (mean±s.d.)], while HPFs ( n =3) exhibited 81.36±5.63% CK − /VIM + cells. Scale bars: 200 µm (A); 50 µm (B).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_9959/pmc12919959/pmc12919959__dmm-19-052436-g1.jpg)